果冻影院

The research published in Molecular Biology and Evolution reports the genome sequence of a malaria parasite sourced from blood-stained medical microscope slides used in 1944 in Spain, one of the last footholds of malaria in Europe. Malaria was a major disease throughout Europe since Antiquity and was only eradicated in the 20th century.

The international team, led by 果冻影院, the Institute of Evolutionary Biology, Barcelona, and the University of Copenhagen, analysed microscopy slides from the 1940s that were obtained with permission from the medical collection of Dr Ildefonso Canicio, a Spanish malaria researcher from the early 1900s. The slides were used to diagnose patients suffering from malaria in Spain鈥檚 Ebro Delta, where malaria was common until the 1960s.

By comparing the genetic data from the slides to a global dataset of modern P. vivax genomes, the researchers found that the eradicated European malaria parasites were genetically most similar to tertian (P. vivax) malaria strains currently found in the Americas, including Mexico, Brazil and Peru.

鈥�鈥淏eing able to obtain a full genome of extinct European Plasmodium vivax from these decades old slides allowed us to ask questions as to how malaria may have been affecting us centuries ago鈥�, said Dr Lucy van Dorp (果冻影院 Genetics Institute). 鈥淲e found a clear relationship with modern Central and South American strains, establishing historic links spreading disease between these continents鈥�.

Analysing a historical sample also enabled the researchers to estimate mutation rates, helping them to infer when the different regional strains of P. vivax malaria diverged from each other. They estimated the last common ancestor between the eradicated European strain and the ones still present in the Americas to the 15^th century.

This divergence is in line with European colonists introducing tertian malaria into the Americas and suggests indigenous peoples of the Americas were not infected before their contact with Europeans. There is no reliable evidence of malaria in the Americas before colonial times, but there are historical accounts of tertian malaria in Europe as far back as classical Greece.

鈥�鈥淲e could date the age of the spread to the Americas to around the 15th century, which clearly points to an introduction of the disease following European contact,鈥� explained co-author Professor Francois Balloux (果冻影院 Genetics Institute).

The researchers were also able to gain new insights into how infectious disease agents can develop resistance to treatments. The team found that the 1940s malaria sample already had some genetic mutations which are known to confer resistance to modern anti-malarial drugs, despite them not having been in use at the time.

The findings suggest drug resistance potential may have already existed in some past malaria strains, possibly due to the historical use of quinine (which has been used to treat malaria as well as other ailments), allowing the parasite to evade modern medications soon after their introduction.

Professor Carles Lalueza-Fox, paleogeneticist at the听听in Barcelona who co-led the study, is excited by the prospect of historic genomes to help us understand malaria. 鈥淢y initial motivation to study this ancient malaria strain is the fact that my father contracted malaria in 1938, while crossing the Ebro region with the Republican army during the Spanish Civil War. After realising the potential of old medical material to understand modern infectious diseases, I got hooked and we鈥檙e currently sourcing more slides from medical and museum collections to understand where malaria emerged first and then spread to other regions of the world鈥�.

The research was supported by the 鈥渓a Caixa鈥� Foundation, FEDER-MINECO, the European Research Council, the MRC and the BBSRC.

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Notes to Editors

Lucy van Dorp, Pere Gelabert, Adrien Rieux, Marc de Manuel, Toni de-Dios, Shyam Gopalakrishnan, Christian Car酶e, Marcela Sandoval-Velasco, Rosa Fregel, I帽igo Olalde, Ra眉l Escosa, Carles Aranda, Silvie Huijben, Ivo Mueller, Tom脿s Marqu猫s-Bonet, Fran莽ois Balloux, M. Thomas P Gilbert and Carles Lalueza-Fox, 鈥�Plasmodium vivax Malaria viewed through the lens of an eradicated European strain鈥� has been published in Molecular Biology and Evolution.

The DOI for this paper will be .